Intermediate

Part:BBa_K359008:Design

Designed by: Cveta Manassieva   Group: iGEM10_Waterloo   (2010-09-02)


AIP generator and sensor, with an added RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2115
    Illegal NheI site found at 2138
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 426
    Illegal BamHI site found at 1310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3732
    Illegal AgeI site found at 3844
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

The sources for this part are Cambridge 2007, creating I746104 - a P2 promoter in agr operon from S. aureus and I13507 which is a screening plasmid intermediate. https://parts.igem.org/cgi/partsdb/add_part_c.cgi

References